Oleh: Endang S. Rahayu
Abstract
Based on FAO/WHO Probiotic is live microorganisms which when administered in adequate amounts confer a health benefit in the host. Speciation of bacteria strain identity must be established using the most current valid methodology. Strain identity was important to link a strain to a specific health effect as well as to enable accurate surveillance and epidemiological studies. It was recommended that a combination of phenotypic and genetic tests must be used. Besides, nomenclature of the bacteria must conform to the current, scientifically recognized names.
In 2013, there were several lactic acid bacteria strains isolated from traditional fermented foods. Some of them identified as Lactobacillus plantarum. In addition, some of these strains were screened for probiotic candidate. A another study found that there were Lactobacillus which potential for probiotic agent, were isolated from intestinal material of healthy infant baby. Based on morphological, biochemical, and physiological characters, several of them were identified as Lactobacillus acidophilus. Based on the studies, sequence of 16s rRNA and RecA gene profile were identified to distinguish species on Lactobacillus plantarum group, as well as pepR gene profile to distinguish species on Lactobacillus casei group. Based on another method, strains Mut-7, Mut-13, T-3, Dad-13 were identified as Lactobacillus plantarum with similarity >99% while SNP-2 was identified as Lactobacillus paracasei. Food and Nutrition Culture Collection (FNCC) code of SNP-2, Mut-7, Mut-13, T-3, and Dad-13 was FNCC 0139, FNCC 0140, FNCC 0141, FNCC 0142, and FNCC 0143, respectively. Recent study revised new nomenclature of Lactobacilli. Based on the prior research, Dad-13 was revised as Lactiplantibacillus plantarum subsp. plantarum Dad-13 while SNP-2 became Lacticaseibacillus paracasei subsp. paracasei SNP-2.
*This poster was presented in The 6th International Conference of Indonesian Society for Lactic Acid Bacteria and Gut Microbiota (6th ISLAB) on 13th August 2021
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